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Figure 1 | BMC Cell Biology

Figure 1

From: Sec61β, a subunit of the Sec61 protein translocation channel at the Endoplasmic Reticulum, is involved in the transport of Gurken to the plasma membrane.

Figure 1

Sec61β protein and Gurken mRNA in ovaries from wild type and sec61βP1germline clones. A) Ovaries were dissected from flies and lysed in buffer containing 2% SDS. Western blotting was performed using Sec61β antibody. A cross-reactive band serves as a loading control. B) Schematic representation of a stage 9–10 egg chamber with the oocyte abutting the 15 nurse cells and surrounded by a layer of somatic follicle cells. The dorsal/anterior corner is depicted in dark red. C-D) Drosophila egg chambers were processed for gurken RNA in-situ hybridization in wild type oocytes, C) and in oocytes mutant for Sec61β, D). n represents the oocyte nucleus.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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