Figure 6

Analysis of the expression and effects on cell growth of various mutated EGFP-RIG1 fusion proteins. (A) Western blot analysis of the mutated EGFP-RIG1 proteins. HtTA cells plated in 6-cm dishes were transiently transfected with 1.5 μg of indicated expression vector for 24 h. (B) Mutated EGFP-RIG1 fusion proteins induced cell death. HtTA cells were transfected with 0.3 μg of the control EGFP or indicated EGFP-tagged wild type or mutated RIG1 expression vectors for 24 or 48 h. Cell death was detected by measuring LDH release, and cell viability was measured with the MTT method. Representative results of three independent experiments are shown and are expressed as means and standard errors of the means after normalization to the control group. wt, wild type; Student t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001.