Figure 6

Convergence-extension is unaffected in cep70 morphants. (A-F) Defective epiboly in cep70 morphant (cep131 morphants normal at this stage). At 70% epiboly, morphant embryos (B) have an acorn shape, unlike normal development at this stage (A). As epiboly completes, morphant embryos (D) (WT shown in C) extrude some of the yolk but will recover and survive. Assaying convergence-extension by the distance between anterior neural margin and prechordal plate by wholemount in situ hybridisation for dlx3 (purple) and hgg1 (light blue) reveals no difference between morphant (F) and WT (E). Scale bar: 125 μm.