HAC colocalization with the chromocenters. Top panels: cells from each of the murine HAC clones were hybridized with 17α (green signal) and minor satellite probes (red signal). In this experiment, the chromocenters are identified by the typical high staining with DAPI (blue). In some cells the HAC do not colocalize with the chromocenters (white arrow), while in other cells there may be partial (orange arrow) or complete colocalization (yellow arrow). The bottom panels display the same cells, stained with DAPI to show the chromocenters distribution, and the outline of the HAC in blue (black arrow).