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Figure 1 | BMC Cell Biology

Figure 1

From: Characterization of the interaction between Actinin-Associated LIM Protein (ALP) and the rod domain of α-actinin

Figure 1

Characterization of ALP107-273 protein. A) Domain architecture of seven human PDZ_LIM proteins. B) SDS-PAGE of the proteins used in SPR measurements. R2-R3 denotes α-actinin spectrin repeats 2–3, R1-R4 spectrin repeats 1–4. ALP denotes the ALP107-273 fragment. Labelled ALP107-273 used in NMR experiments had a similar purity to the non-labelled form. C) Size exclusion chromatography showed that ALP107-273 eluted faster than expected based on its calculated monomer molecular weight (18 265 Da).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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