Figure 4
Immuno-staining of lung sections for proSP-C. A. Sections of frozen lungs from Control and ASCFTR rats were probed with polyclonal antibodies to proSP-C and then with Cy3-labeled secondary antibodies (red). The cell nuclei were counter-stained with DAPI (blue). The sections were viewed with a fluorescence microscope and photographed after deconvolusion and normalization of Cy3 fluorescence intensity to the same range of pixel density for the Control and ASCFTR specimens. B. A comparison of fluorescent object count between the Control and ASCFTR specimens showed a significant decrease in proSP-C positive cells (ATII cells) in the ASCFTR group in comparison to the Control group.