Table 2 Strains used in this study. All strains are W303 background.
From: Bub2 regulation of cytokinesis and septation in budding yeast
Strain Name | Genotype | Source |
|---|---|---|
KSY3 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbar1 | Lippincott and Li, 1998 (RLY261) |
KSY19 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbub2:LEU2 Δbar1 | Li lab (RLY597) |
KSY21 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 Δbub2:LEU2 | Li, 1999 (RLY684) |
KSY22 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 Δmad2:LEU2 | Li, 1999 (RLY686) |
KSY24 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δmad2:LEU2 | Li, 1999 (RLY718) |
KSY25 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbub2:LEU2 Δmad2:URA3 | Li, 1999 (RLY736) |
KSY47 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbub2:LEU2 Δmad2:URA3 Δbar1:HIS3 | This work |
KSY51 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δmad2:LEU2 Δbar1:HIS3 | This work |
KSY70 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbar1 MYO1-GFP: TRP1 (pKT36) | This work |
KSY71 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbub2:LEU2 Δbar1 MYO1-GFP: TRP1 (pKT36) | This work |
KSY73 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δmad2:LEU2 Δbar1:HIS3 MYO1-GFP: TRP1 (pKT36) | This work |
KSY110 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 Δbub2:LEU2 Δbar1:URA3 | This work |
KSY111 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 Δmad2:LEU2 Δbar1:URA3 | This work |
KSY129 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbar1 GAL1-T7-BUB2-6His: URA3 (pKL1315) | This work |
KSY130 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbub2:LEU2 Δbar1 GAL1-T7-BUB2-6His: URA3 (pKL1315) | This work |
KSY132 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbar1 MYO1-GFP: TRP1 (pKT36) GAL1-T7-BUB2-6His: URA3 (pKL1315) | This work |
KSY167 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 | Li, 1999 (RLY683) |
KSY169 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbfa1: HIS3 GAL1-T7-BUB2-6His: URA3 (pKL1315) | This work |
KSY171 | MATa ura3–52 leu2–3,112 his3-Δ200 trp1-1 ade2 Δbar1 GAL1-T7-BUB2-6His: URA3 (pKL1315) GAL1-myc-Tem1 (pKT25) | This work |