Figure 4

Counter-staining analyses using fluorometric DNA and RNA dyes. A) Counter-staining with DNA dyes demonstrates the sensitivity, permeability and the adequate signal to noise ratio of RNA dyes. Hoechst staining has a significant amount of background compared to 132A. 132A detected parasites that were overlooked by DAPI staining. B) Counter staining with nucleic acid dye DAPI, in red, and RNA dyes 107F, 107E, and 132A, in green, at schizont, trophozoite, and ring stages of erythrocytic cycle. Images obtained by the Pathway HT were grayscale; red and green colors were artificially added to emphasize localization.