Table 3 Mutants analyzed in this study
Mutant/WT | Sequence | Rescue |
|---|---|---|
SBH1P TM | MLRVDPLVVLFLAVGFIFSVVALHVIS | Yes |
SBH1P TM SP1 | MLRVDS LVVLFLAVGFIFSVVALHVIS | Yes |
SBH1P TM SP2 | MLRVDPLVG LFLAVGFIFSVVALHVIS | Yes |
SBH1P TM DP | MLRVDS LVG LFLAVGFIFSVVALHVIS | No |
SBH1P TM mutant 1 | MLRVDPLVVLFLAVGFTS SVVALHAT S | Yes |
SBH1P TM mutant 2 | MLRVDPLVVLFLAVGFIFSVVS LHVIS | Yes |
SBH1P TM mutant 3 | MLRVDPLVVLL LAA GFIL SVA AS HVT S | Yes |
SBH1P TM mutant 4 | MLRVDPLVVLFP AVGFIFSVVALHVIS | Yes |
SBH1P TM mutant 5 | MLRVDPLVVLFLAVGS IFP VVALR VIS | No |
SBH1P TM mutant 6 | MLRVDPLVVLS LAVGFV FP VVALHVIS | No |
SBH1P TM mutant 7 | MLRVDPLVA LFLAVGFN FSVVAP HVIS | No |
SBH1P TM mutant 8 | MLRVDPLVE LFP AVGFIS SVVALHVIS | No |
SBH1P TM mutant 9 | MLT VDPLVVLFLAVGFIL SVVALHVIS | Yes |
SBH1P TM mutant 10 | MLRVDPLVG LL LAVGFIL SVVALHVIS | Yes |
SBH1P TM mutant 11 | MLRVDPLVVLFLAVGFIP SVA ALHVIS | Yes |
SBH1P TM mutant 12 | MLRVDPLA VS FLAA GFIP SVVALHVIS | Yes |
SBH1P TM mutant 13 | MLRVDPLA VLFP AVGFIY SVVALHVIS | Yes |
SBH1P TM mutant 14 | MLRVDPLVVLFLAVGFT FSVVALHVIS | Yes |
SBH1P TM mutant 15 | MLRVDPS VVLFLAVGL IFSVVALR VIS | Yes |
SBH1P TM mutant 16 | MPS VDPLVVLFP AVGFIFSVVALHVIS | Yes |
SBH1P TM mutant 17 | MLRVDPLVVLFLAVGFV FSVVALHVIS | Yes |
SBH1P TM mutant 18 | MLRVG PLVVLFLAVGFIFSVVALHVIS | Yes |
SBH1P TM mutant 19 | MLRVDPLVVLFLAVGS IFSVVALHVIS | Yes |
SBH1P TM mutant 20 | MLRVDPLVVLFLAVGFIFP VVALHVIS | Yes |
SBH1P TM mutant 21 | MLRVG PLVVLFLAVGL IFSVA ALR VIS | No |
SBH1P TM mutant 22 | MP RVDPLVVLS LAVGFV FP VVALHVIS | No |
SBH1P TM mutant 23 | MLRA DPLVA LFLAVGS IFSVVALHVIS | No |
SBH1P TM mutant 24 | MP RVDPLVE LFP AVGFIS SVVALHVIS | No |
SBH1P TM mutant 25 | MP RVDPLVA LFLAVGFN FSVVAP HVIS | No |
SBH1P TM mutant 26 | MLRVDPLVA LFLAVGLT FSVVAY RYFL | No |
SBH1P TM mutant 27 | MLRVDPLAAS FP AVGSYLP VA ALHVIS | No |
SBH1P TM mutant 28 | MLRVDPLVASS LAVGLV FP VVALHVIS | No |