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Table 3 Mutants analyzed in this study

From: Functional characterization of the trans-membrane domain interactions of the Sec61 protein translocation complex beta-subunit

Mutant/WT Sequence Rescue
SBH1P TM MLRVDPLVVLFLAVGFIFSVVALHVIS Yes
SBH1P TM SP1 MLRVDS LVVLFLAVGFIFSVVALHVIS Yes
SBH1P TM SP2 MLRVDPLVG LFLAVGFIFSVVALHVIS Yes
SBH1P TM DP MLRVDS LVG LFLAVGFIFSVVALHVIS No
SBH1P TM mutant 1 MLRVDPLVVLFLAVGFTS SVVALHAT S Yes
SBH1P TM mutant 2 MLRVDPLVVLFLAVGFIFSVVS LHVIS Yes
SBH1P TM mutant 3 MLRVDPLVVLL LAA GFIL SVA AS HVT S Yes
SBH1P TM mutant 4 MLRVDPLVVLFP AVGFIFSVVALHVIS Yes
SBH1P TM mutant 5 MLRVDPLVVLFLAVGS IFP VVALR VIS No
SBH1P TM mutant 6 MLRVDPLVVLS LAVGFV FP VVALHVIS No
SBH1P TM mutant 7 MLRVDPLVA LFLAVGFN FSVVAP HVIS No
SBH1P TM mutant 8 MLRVDPLVE LFP AVGFIS SVVALHVIS No
SBH1P TM mutant 9 MLT VDPLVVLFLAVGFIL SVVALHVIS Yes
SBH1P TM mutant 10 MLRVDPLVG LL LAVGFIL SVVALHVIS Yes
SBH1P TM mutant 11 MLRVDPLVVLFLAVGFIP SVA ALHVIS Yes
SBH1P TM mutant 12 MLRVDPLA VS FLAA GFIP SVVALHVIS Yes
SBH1P TM mutant 13 MLRVDPLA VLFP AVGFIY SVVALHVIS Yes
SBH1P TM mutant 14 MLRVDPLVVLFLAVGFT FSVVALHVIS Yes
SBH1P TM mutant 15 MLRVDPS VVLFLAVGL IFSVVALR VIS Yes
SBH1P TM mutant 16 MPS VDPLVVLFP AVGFIFSVVALHVIS Yes
SBH1P TM mutant 17 MLRVDPLVVLFLAVGFV FSVVALHVIS Yes
SBH1P TM mutant 18 MLRVG PLVVLFLAVGFIFSVVALHVIS Yes
SBH1P TM mutant 19 MLRVDPLVVLFLAVGS IFSVVALHVIS Yes
SBH1P TM mutant 20 MLRVDPLVVLFLAVGFIFP VVALHVIS Yes
SBH1P TM mutant 21 MLRVG PLVVLFLAVGL IFSVA ALR VIS No
SBH1P TM mutant 22 MP RVDPLVVLS LAVGFV FP VVALHVIS No
SBH1P TM mutant 23 MLRA DPLVA LFLAVGS IFSVVALHVIS No
SBH1P TM mutant 24 MP RVDPLVE LFP AVGFIS SVVALHVIS No
SBH1P TM mutant 25 MP RVDPLVA LFLAVGFN FSVVAP HVIS No
SBH1P TM mutant 26 MLRVDPLVA LFLAVGLT FSVVAY RYFL No
SBH1P TM mutant 27 MLRVDPLAAS FP AVGSYLP VA ALHVIS No
SBH1P TM mutant 28 MLRVDPLVASS LAVGLV FP VVALHVIS No
  1. Black color highlights the mutated amino acid