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Table 1 Changes in [Ca2+]i in response to CCK-8 alone and in the presence of ethanol, and effect of alcohol dehydrogenase inhibition by 4-MP and cinnamtannin B-1

From: Ethanol exerts dual effects on calcium homeostasis in CCK-8-stimulated mouse pancreatic acinar cells

  CCK-8 CCK-8/EtOH CCK-8/EtOH/4-MP CCK-8/EtOH/CinB
Peak [Ca2+]i (nM) 314.30 ± 17.39 n = 17 462.10 ± 22.26 n = 14
***
469.40 ± 15.30 n = 7
***
448.00 ± 19.99 n = 11
*
Steady level (nM) 136.30 ± 7.75 n = 17 173.10 ± 9.76 n = 14
**
140.10 ± 9.64 n = 7
134.40 ± 11.21 n = 11
††
Rate of decay
(Δnmol/s)
0.0093 ± 0.0014 n = 17 0.0051 ± 0.0004 n = 14
*
0.0105 ± 0.0014 n = 7
††
0.0077 ± 0.0006 n = 11
††
Total Ca2+ (nM) 27930 ± 2801 n = 17 43380 ± 4844 n = 14
**
37470 ± 2010 n = 7
*
39610 ± 5163 n = 11
*
  1. Pancreatic acinar cells were stimulated with 1 nM CCK-8 alone, in the presence of 50 mM ethanol (EtOH), after preincubation in the presence of 1 mM of the alcohol dehydrogenase inhibitor 4-MP or following preincubation in the presence of 10 μM of the antioxidant cinnamtannin B-1 (CinB). Following CCK-8 stimulation, the peak [Ca2+]i response, the "steady-state level" achieved five minutes after application of the stimulus, the total Ca2+ mobilization and the rate of decay of [Ca2+]i towards basal values were calculated (*, P < 0.05; **, P < 0.01; ***, P < 0.001 vs CCK-8; †, P < 0.05; ††, P < 0.01 vs CCK-8 plus EtOH).