Figure 6From: Disruption of occludin function in polarized epithelial cells activates the extrinsic pathway of apoptosis leading to cell extrusion without loss of transepithelial resistancePeptide localization in EPH4 cells. EPH4 cells grown on glass slides were treated with FITC labeled LYHY (A) or LYQY (B) peptides (350 μM) for 6 hr (pseudocolored red). After fixation, cells were stained with antibodies against occludin (green pseudocolor) and E-cadherin (green pseudocolor) and with DAPI for nuclei (blue). LYHY accumulated in regions enriched in non-junctional occludin, denoted by arrowhead. LYQY did not alter occludin distribution although it appeared to localize in the cytoplasm of some cells. E-cadherin distribution was not altered by LYHY.Back to article page