Morphological and functional analysis of mitochondria in cells lacking α1,6-mannosyltransferase activity. (A) DASPMI staining of Kloch1-1 cells and the parental strain. (B) GFP fluorescence of mitochondrial matrix of wt and mutant strains harbouring p426SD11 mtGFP vector. (C) Mitochondrial analysis by electron microscopy of Kloch1-1 cells and the parental strain. Cultures were grown to exponential phase into YPD medium. n, nucleus; m, mitochondrion; cw, cell wall; bar 2 μm.