Phenotypical analysis of Kloch1-1 overexpressing KlCNB1 or both subunits of calcineurin. (A) DASPMI staining of the Kloch1-1 cells harbouring pKlCNB1 plasmid alone or with the pKlCNA1 plasmid. (B) Ultra-thin sections of above strains. n, nucleus; m, mitochondrion; cw, cell wall; bar 2 μm. (C) Serial dilutions of cultures from the same strains onto YPD agar plates supplemented with the cell wall interfering agent calcofluor white (CFW). The growth at 28°C was monitored after 3 d; three independent transformants have been checked, obtaining identical results.