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Table 1 Replication factory numbers determined by confocal and STED microscopy.

From: Analysis of replication factories in human cells by super-resolution light microscopy

 

Confocal

STED

 

Mean

Range

Mean

Range

PCNA -HU

1.6

1.2 - 2.1

4.1

2.1 - 5.4

RPA -HU

2.8

1.0 - 4.1

4.7

3.0 - 7.6

PCNA +HU

1.7

1.4 - 1.8

2.6

1.5 - 3.5

RPA +HU

1.8

1.2 - 2.6

2.8

1.9 - 3.9

  1. Cells treated with HU or untreated were analysed for RPA or PCNA containing foci by indirect immunofluorescence using confocal or STED microscopy. The images were restored using the CMLE algorithm and rendered in 3D using SVI's Huygens software, which was also used to count the objects. The average number of objects per μ m3 (n = 3-5) and the range of values found are given for a garbage threshold volume (below which size objects are not counted) of 5 voxels (corresponding to 0.0003 μ m3).