The autophagy inhibitor rescued tBHP-induced cell death in Bβ2 clones. (A) Cell growth determination A total of 1 × 105 exponentially growing cells were cultured with either 10 or 20 μM of 3-MA before being treated with 50 nM of tBHP for 48 h. The cells were then trypsinized and counted for viable cells by trypan-blue staining. The results represented averages of three independent experiments and error bars standard errors. (B) tBHP-induced apoptotic cell death can be blocked by 3-MA Annexin V binding in Bβ2 clones was determined by flow cytometry 48 h after treatment with 50 nM of tBHP in the presence and absence of 10 or 20 μM of 3-MA, respectively, using SK-N-SH cells as control. The results represented averages of three independent experiments and error bars standard errors. Data are represented as the mean ± standard errors of three independent experiments, each performed in duplicate. *P < 0.05, significance of difference as compared with the control group.