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Table 1 Effect of various substances on the cell surface expression of wild type (HA-WT) and mutant (HA-MM) HA-ABCA1 variants

From: Model system for the analysis of cell surface expression of human ABCA1

Drug

Known effect

ABCA1 cell surface expression

  

HA-WT

HA-MM

ALLN

Calpain inhibitor

(+ 60%)

(+ 30%)

ApoA-I

cholesterol acceptor

(+ 30%)

no effect

Atorvastatin

cholesterol level lowering

no effect

no effect

Brefeldin A

trans-Golgi transport inhibitor

(- 60%)

(- 40%)

Cycloheximide

protein synthesis inhibitor

(- 30%) (viability )

(- 30%) (viability )

Cyclosporin A

immunosuppressor

(+ 60%)

(+ 60%)

Ezetimibe

inhibitor of intestinal cholesterol absorption

(- 20%)

no effect

Glyburide

ABCA1 inhibitor

no effect

no effect

Niacin

cholesterol level lowering

no effect

no effect

Nifedipine

Ca2+-channel blocker

no effect

no effect

Methyl-β-cyclodextrin

cholesterol depletion

no effect (viability )

no effect (viability )

Cholesterol-methyl-β-cyclodextrin

cholesterol overloading

no effect (viability )

no effect (viability )

Verapamil

Ca2+-channel blocker

no effect

no effect