Figure 4
Regulation of 20E in the expression of Ha-Ntf2 or Ha-Ran. A: RT-PCR analysis the regulation of 20E in the expression of Ha-Ntf2. B: Regulation of 20E in the expression of Ha-Ran. Other signs are: C stands for the normal 6th-0 h larvae; D1, D3, D6, D12 and D24 are 1, 3, 6, 12 and 24 h after injected by the DMSO, respectively; E1, E3, E6, E12 and E24 stand for 1, 3, 6, 12 and 24 h after injected by the 20E, respectively. β-actin is used as a quantitative control. Error bars represent the standard deviation in three independent experiments. An asterisk indicates significant differences (Student's t-test, *: p < 0.05).