Figure 7
Variation of the subcellular localization of EcR-B1 in the HaEpi cell after the knockdown of Ha-Ntf2 or Ha-Ran. Panel A': The EcR-B1 from the HaEpi cell extract could be detected by the monoclonal antibody of EcR-B1 from the M. sexta using immunoblotting. Panels A-F: The red color indicates EcR-B1 stained with anti-EcR-B1 monoclonal antibody; the blue color indicates nuclei counterstained with DAPI. Neg, negative control without first antibody; nor, normal cells cultured in Grace's medium; nor+20E, the cells cultured in Grace's medium plus 20E; dsGFP+20E, the cells cultured in Grace's medium plus dsGFP and 20E; dsRan+20E, the cells cultured in Grace's medium plus dsRan and 20E; dsNtf2+20E, the cells cultured in Grace's medium plus dsNtf2 and 20E. Nu: nucleus; Cy: cytoplasm. Size bars = 50 μm.