Figure 3

Analysis of cell type-specific markers in NI-hADSCs. Human ADSCs were induced to differentiate into neural cells in the presence of bFGF and forskolin over two weeks. a, Fluorescent immunocytochemistry revealed that the expression of nestin, Tuj1, MAP2, NFL, NFM, NFH, NSE, NeuN, GAP43, SNAP25, GFAP, and CNPase in NI-hADSCs increased more than those of primary hADSCs. Scale bars measure 50 μm. b, Immunocytochemical data depicts the high ratio of NI-hADSCs expression above neuronal markers. The number of positive cells was counted and the ratio of positive cells to nuclei was analyzed for each antigen (n = 7). c and d, RT-PCR analysis demonstrated increased mRNA expression for ABCG2, nestin, Tuj1, MAP2, NFL, NFM, NSE, GAP43, SNAP25, GFAP and CNPase genes. GAPDH was used as a control. The intensity of each gene was normalized to GAPDH and these results were repeated at least five times. * P < 0.05, ** P < 0.01 compare with the primary hADSCs