Table 1 Antibodies used in the study.
Antibody | Isotype | Suppliers | Cat. No | Dilution |
|---|---|---|---|---|
α-SMA | Mouse IgG2a | Sigma | A2547 | 1:200 |
E-cadherin | Mouse IgG2a | BD Biosciences | 610182 | 0.25 μg/ml |
TGF-β1 | mouse IgG1 | R&D Systems | MAB240 | 5 μg/ml |
TβR-I | Rabbit IgG | Cell Signaling Technology | #3712 | 1:1000 |
Smad2 | Mouse IgG1 | Cell Singaling Technology | #3103 | 1:800 |
Smad3 | Rabbit IgG | Zymed Laboratories | 51-1500 | 1:200 |
CK-18 | Mouse IgG1 | Chemicon International | MAB1600 | 1:200 |
p-Smad2 | Rabbit IgG1 | Cell Singaling Technology | #3101 | 1:200 |
p-Smad3 | Rabbit IgG1 | Cell Singaling Technology | #9520S | 1:200 |
Smad3 | Rabbit IgG | Epitomics | 1735-1 | 1:2000 |
p-Smad3 | Rabbit IgG | Epitomics | 1880-1 | 1:1000 |
Smad2 | Rabbit IgG | Epitomics | 1736-1 | 1:1000 |
p-Smad2 | Rabbit IgG | Abcam | Ab53100 | 1:300 |
Smad7 | Rabbit IgG | Santa Cruz Biotechnology | SC-11392 | 1:100 |