Figure 3

Centrioles migrate occasionally towards the intercellular bridge during cytokinesis in human epithelial cells. Representative images of centriole migration to the intercellular bridge prior to abscission (first 3 time points) and after abscission (t = 90 min) are shown for the human MCF 10A epithelial cell line (see Additional file 3 movie 2). The cells were transiently co-transfected with centrin1-EGFP (green) and α-Tubulin-mCherry (red) expression constructs, to label centrioles and the microtubules, respectively. White arrows point at a centriole migrating to the intercellular bridge.