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Figure 2 | BMC Cell Biology

Figure 2

From: Prolonged activation of S6K1 does not suppress IRS or PI-3 kinase signaling during muscle cell differentiation

Figure 2

Differentiation decreases p42/44 and PKB signaling whereas S6K activity increases. A) p44/42 MAPK Thr202/Tyr204 phosphorylation relative to total p44/42 (p-p44/42), B) PKB Thr308 phosphorylation relative to total PKB (p-PKB) and C) S6K1 phosphorylation and activity determined relative to total S6K1 3 hrs post plating (subconfluent; SC), 24 h post plating (Day 0), 24 h after achieving confluence (Day 1), or 72 hrs following differentiation (Day 3). * indicates significantly different from SC, † indicates significantly different from Day 1. Values were obtained by normalising specific phosphorylation to total protein content as described in methods.

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