Figure 2

Arecoline downregulates of ZO-1 and HER2 transcripts in Ishikawa cells. Ishikawa cells were treated with the DMSO vehicle control, 0.1 mM, 0.3 mM or 0.5 mM arecoline for 48 hrs and total RNA was isolated and quantified by RT-PCR analysis. Oligonucleotides specific for ZO-1, HER2 or GAPDH were used to generate specific RT-PCR fragments that were fractionated in agarose gels. The transcript specific bands were visualized by ethidium bromide staining.