Figure 1

Isolation and validation of PAs interacting with MT1-MMP ICD. (A) Schematic representation of the TrxA scaffold and the PAs swiggle, 76, and s14. The sequences of the peptide inserted in TrxA (swiggle, s14) or fused to the N-terminal region of TrxA (76) are detailed. The point mutation in the sequence of s14 is depicted in bold and the amino acids in lower cases in PA 76 represent linkers between the multiple peptides. * denotes the stop codon. (B) Swiggle interacts with the MT1-MMP ICD in a yeast-two hybrid interaction assay. EGY48 cells expressing LexA-DBD, LexA-MT1, LexA-MT2, LexA-MT3, LexA-MT5 or LexA-Cdk4 were mated with EGY42 cells expressing AD-TrxA, AD-swiggle, AD-s14, AD-76 or AD-CyclinD1 and plated onto selective media.