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Figure 5 | BMC Cell Biology

Figure 5

From: Peptide aptamers as new tools to modulate clathrin-mediated internalisation — inhibition of MT1-MMP internalisation

Figure 5

GFP-swiggle increases MT1-MMP-mediated Texas Red-gelatin degradation. Example of Texas-Red gelatin degradation (white dotted line) by MCF7 cells expressing MT1-MMP and (A) GFP-s14 or (B) GFP-swiggle after 16 hours incubation at 37°C. GFP-s14 or GFP-swiggle (green) and MT1-MMP (purple) expression were detected by immunofluorescence. Bar = 20 μm (C) Quantification of the area of migration of at least 50 untransfected MCF7 cells or 50 cells transfected with GFP-s14, GFP-swiggle, MT1-MMP, MT1-MMP + GFP-s14, MT1-MMP + GFP-swiggle, MT1-MMP E240A, MT1-MMP E240A + GFP-s14, MT1-MMP E240A + GFP-swiggle, MT1-MMP ΔICD, MT1-MMP ΔICD + GFP-s14 or MT1-MMP ΔICD + GFP-swiggle. Data represent average area of migration ± s.e.m..

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