Figure 8
Swiggle interacts with the LLY573 motif of the MT1-MMP ICD. (A) EGY48 cells expressing LexA-DBD, LexA-MT1, the LexA-MT1 LLY/A mutant or LexA-Cdk4 were mated with EGY42 cells expressing AD-swiggle, AD-s14 or AD-CyclinD1 and plated onto selective media. (B) Cell lysates prepared from MCF7 cells expressing MT1-MMP alone (lanes 1 and 2) or together with GFP-s14 (lane 3) or GFP-swiggle (lane 4) were subjected to immunoprecipitation in the presence (lanes 2, 3 and 4) or the absence (lane 1) of an anti-MT1-MMP pAb The presence of μ2 and MT1-MMP in the immunocomplexes was monitored by Western blotting. The expression level of GFP-s14, GFP-swiggle or μ2 in the input lysates was analysed by Western blot.