Figure 9

Inhibiting Herp expression increases insulin 2 (C96Y)-EGFP levels and enhances apoptosis. A. Clone #4S2 cells were untreated or treated with 2 μg/ml doxycycline for the times indicated and cell lysates were prepared and immunoblotted. B. Clone #4S2 cells were transfected with control siRNA or an siRNA directed against Herp and treated or not with doxycycline for 48 h. Cells were lysed and immunoblotted as indicated. A low and high exposure of the anti-GFP western blot is shown. Results from two of four independent experiments are shown. C. Immunoblots were quantified and the mean relative expression of Herp from 4 independent experiments is presented. D. Clone #4S2 cells were transfected with control or Herp siRNA and 48 h later apoptosis was measured by immunobloting for cleaved caspase 3. Results from two experiments are shown. E. Clone #4S2 cells were transfected as in (D) and 24 h later doxycycline was added or not for 48 h and apoptosis was measured by immunobloting for cleaved caspase 3. INS-1 cells treated or not with 0.3 mM straurosporine (St.) was included as a positive control for cleaved caspase 3.