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Table 1 Down regulation of ASPM in response to siRNA mediated ASPM depletion in metaphase U2OS cells.

From: Human ASPM participates in spindle organisation, spindle orientation and cytokinesis

 

GL3

ASP1

ASP2

Average integral intensity (μm2)

14805

2318

6631

Knockdown of spindle pole expression (%)

0

84.3

55.2

p value

 

<0.001

0.005

  1. Comparative immunofluorescence analysis, measured as average integral intensity, was performed on the immunofluorescent centrosomal ASPM signal of twenty-five metaphase U2OS cells treated with ASP1, ASP2 or GL3 siRNAs. In comparison to the average integral intensity of ASPM spindle pole signal measured in the GL3 treated metaphases, a significant decrease in the average integral intensity of ASPM signal at the spindle pole was calculated after 72 hr treatment with ASP1, and with ASP2. The knock down is expressed as a percentage of the expression in GL3 cells. In each case t-tests showed the levels of knockdown to be significant.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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