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Table 2 Phospholipid profile of transfected MLE-12 cells stably expressing mutant SP-CI73T.

From: A non-BRICHOS surfactant protein c mutation disrupts epithelial cell function and intercellular signaling

Ā 

WT

I73T

P (Anova)

Total phospholipids (nmol/mg protein)

152.80 Ā± 9.6

153.10 Ā± 10.0

ns

Phospholipid classes (% of total PL)

Ā Ā Ā 

Phosphatidylcholine

57.80 Ā± 1.0

53.70 Ā± 0.8

< 0.001

Lyso-Phosphatidylcholine

0.60 Ā± 0.1

1.00 Ā± 0.8

< 0.001

Phosphatidylglycerol

0.30 Ā± 0.0

0.20 Ā± 0.1

ns

Sphingomyelin

6.20 Ā± 0.3

5.50 Ā± 0.2

< 0.05

Ceramide

1.80 Ā± 0.1

1.80 Ā± 0.1

ns

Glucosyl-Ceramide

0.10 Ā± 0.0

0.10 Ā± 0.0

ns

Phosphatidylethanolamine

11.20 Ā± 0.5

14.70 Ā± 0.8

< 0.001

Phosphatidylserine

6.50 Ā± 0.1

6.60 Ā± 0.3

ns

  1. Data are means of three independent experiments each performed in duplicate. P-values are given.