DNA moves sequentially towards the nuclear matrix during DNA replication in vivo

Table 5 Target sequences corresponding to four unrelated genes from separate chromosomes

Amplicon	Chromosome	Forward Primer	Reverse Primer	Amplicon Length
GFAP	10	TCCAGCCCGTCCCTCAATAA	TCCCGAAGTTCTGCCTGGTAA	418 bp
CD23	12	TAGGAGACGATGCTGCTGTGCA	CGTGGGAAGAGGATCAGACAAGAA	284 bp
MPZ	13	CTTGCCCCTACCCCAGCTAT	TCTCCTTGGCTGGCTCTCAAT	184 bp
Fyn	20	ACACAATGCTGATCTAGTCGTGGC	CACATCTGTGTTCATCACTGTCCG	340 bp

Sets of primers and corresponding amplicon sizes defining target sequences in four unrelated genes located in different rat chromosomes. All the amplicons were designed for the 5' end of the corresponding gene.

ISSN: 2661-8850