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Table 6 Location of the target sequences of unrelated genes relative to the NM

From: DNA moves sequentially towards the nuclear matrix during DNA replication in vivo

  TOPOLOGICAL ZONES RELATIVE TO NM ACCORDING TO KINETICS OF DNA DIGESTION OF NUCLEOIDS FROM GO HEPATOCVTES
Amplicon CONTROL G0   PHX-24   PHX-7D
  C D P VC E   C D P VC E   C D P VC E
Fyn + + - - -   + + + + -   + + - - -
CD23 + + - - -   + + + + -   + + - - -
GFAP + + - - -   + + + + -   + +* - - -
MPZ + + + - -   + + + + -*   + + +* - -
  TOPOLOGICAL ZONES RELATIVE TO NM
Amplicon CONTROL GO   PHX-24   PHX-7D
  C D P VC E   C D P VC E   C D P VC E
Fyn + + - - -   + + + - -   + + - - -
CD23 + + - - -   + + + -* -   + + - - -
GFAP + + - - -   + + + - -   + +* - - -
MPZ + + + - -   + + + + -*   + + +* - -
  1. Location of four target sequences each belonging to a different gene in a separate chromosome, within the topological zones relative to the NM. In the upper section of the table the topological zones were defined according to the kinetics of nucleoid-DNA digestion in samples from control G0 hepatocytes obtained from normal rat liver (Figure 2A). In the lower section of the table the topological zones were defined according to the specific kinetics of nucleoid-DNA digestion for each kind of nucleoid sample (Figure 2A for G0; Figure 2B for PHx-24; Figure 2C for PHx-7D samples). The amplicons were scored either as positive or negative as a function of endonuclease digestion time and for each topological zone relative to the NM, depending on whether or not they were detected by a digital image-analysis system (Kodak 1D Image Analysis Software 3.5) using the default settings. G0, samples from normal rat liver hepatocytes. PHx-24, samples obtained from hepatocytes 24 h after partial hepatectomy. PHx-7 D, samples obtained from hepatocytes 7 days after partial hepatectomy. C, 0' digestion-time control. D, distal to the NM. P, proximal to the NM. VC, very close to the NM. E, embedded within the NM. All target sequences were mapped in repeated experiments with samples from separate animals (n ≥3). *Indicates that one of the repetitions gave a different result: -* in one experiment was positive; +* in one experiment was negative.