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Figure 5 | BMC Cell Biology

Figure 5

From: Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness

Figure 5

Changes in the protein amounts during electrotaxis. Intracellular levels of NaKA (total + phosphorylated), NHE1, NHE3 (total + phosphorylated) and PIP2 in cathodally (Calvaria) and in anodally (SaOS-2) migrating cells (A, B). Error bars represent the SEM for 11-16 separate measurements (*P = 0.01). FACS histograms that represent original measurements of NaKA (total + phosphorylated), NHE1, NHE3 (total + phosphorylated), and PIP2 from cathodally (Calvaria) and from anodally (SaOS-2) migrating cells (C, D). Images from the Western blots which show the osteoblastic isoforms of the NaKA (whole cell lysate, 110 kDa), NHE1 (whole cell lysate, 92 kDa), and NHE3 (immunprecipitate, 85 kDa) used in this study (E, F).

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