Figure 1

Nuclear speckles aggregate during desiccation of the microspore of M. vestita. Bright field microscopy: a-d, Labeling of microspores with SC-35 mAb against nuclear speckle marker SC35. e-h, TBO staining. i-l, ISH with probe for 25S rRNA. m-p, Labeling of microspores with mAb 72B9 against nucleolar marker Fibrillarin. Microspores were harvested from plants that were watered (a, e, i, &m), had not been watered for 2 weeks (b, f, j, &n), had not been watered for 4 weeks (c, g, k, &o) and had not been watered for 6 weeks (d, h, l, &p). Arrows denote subnuclear aggregations. Bar = 25 μm.