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Figure 4 | BMC Cell Biology

Figure 4

From: Protein release through nonlethal oncotic pores as an alternative nonclassical secretory pathway

Figure 4

PGK1 released as a free soluble protein. Media was collected from HeLa cell cultures 7.5 min after serum withdrawal and then treated with (lanes 2 and 4) or without Proteinase K (PK, lanes 1 and 3) in the presence (lanes 3 and 4) or absence (lanes 1 and 2) of Triton X-100 (Triton) as described in Materials and Methods. Samples were separated on SDS-PAGE gels and then immunoblotted with anti-PGK1 antibodies.

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