Figure 1From: Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorterSpecification of Msi1high cells in vitro derived from mouse ESCs. (A): Relative abundance of mRNA of Msi1 in 5-day EBs (EB, red bar) and induction stage cells at 5-day in EGF medium (EGF, solid bar), SF medium (SF, gray bar), and FCS medium (FCS, blank bar) compared with ESCs (ESC, blue bar). Bars show mean ± SE (n = 3) measured by real-time RT-PCR. The red bar indicates that its abundance is 1.0 on these two scales. (*P< 0.05 compared with all other samples) (B): The immunostaining for Msi1 by immunocytochemistry was detected in partial cells at the 5-day differentiation cultured in EGF, SF, and FCS medium. (arrowheads; Bar indicates 20 μm in this panel) (C): Percentage of Msi1+ cells at the 5-day differentiation cultured in EGF, SF, and FCS medium. (Bar shows mean ± SE in this panel; n = 3 individual experiments; *P< 0.05 compared with all other samples).Back to article page