Figure 3

Msi1 expression cells can be enriched in SP fraction. (A): SP and NSP fractions were synchronically sorted from the Msi1^high cells by FACS. (B): Relative abundance of mRNA of Msi1 in SP and NSP fractions sorted from Msi1^high cells compared with unsorted Msi1^high cells. Bars show mean ± SE (n = 3) measured by real-time RT-PCR. The mRNA abundance in unsorted Msi1^high cells is 1.0 on these two scales. (*P< 0.05 compared with all other samples) (C): Expressions of the Msi1 and β-actin proteins in SP and NSP fractions sorted from Msi1^high cells detected by Western blots. (D): Protein levels of Msi1 indicated by the integrated intensities of corresponding bands in panel C (Mean ± SE values, n = 3 individual experiments). (*P< 0.05 compared with all other samples; N.D indicates not detected).