Skip to main content
Figure 4 | BMC Cell Biology

Figure 4

From: Ubiquitination is involved in secondary growth, not initial formation of polyglutamine protein aggregates in C. elegans

Figure 4

Q82::GFP aggregates contain both K48- and K63-linked polyubiquitin chains. Synchronized adult worms were fixed and probed with antibodies specific for K48 or K63 polyubiquitin chains. Secondary antibody was conjugated to TRITC (A, D, and G). Aggregates were visualized via GFP fluorescence (B, E, H). Control samples (G) received the same treatment, without primary antibody. Merged images (C, F) show colocalization of K48- or K63-linked polyubiquitin chains to aggregates, but not when the primary antibody is omitted (I).

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us