Figure 3

Subcellular localization of GFP-KIAA1377. HeLa cells transfected with GFP-KIAA1377 (green) were fixed and probed with immunofluorescence. Images at single focal planes are shown. Bar = 10 μm. (A&B) Transfected cells finishing cytokinesis were co-stained with anti-α-tubulin (red in A) or anti-Plk1 antibody (purple in B) and counter-stained with DAPI for DNA (blue). (C) A transfected cell expressing higher level of GFP-KIAA1377 (green) was co-stained for α-tubulin (red) and DNA (blue) to indicate co-localization of GFP-KIAA1377 with microtubule structures. (D) GFP-KIAA1377 is localized at centrosomes. Cells at different stages of cell cycle are distinguished based on anti-centrin 2 (red) and DAPI (blue) staining. The GFP signals in different cells were adjusted to the same scale. Enlarged insets show details of boxed areas