Figure 3

Fusion index in terminally differentiated primary avian and mammalian muscle cultures. Each species displayed a significantly different fusion index (p < 0.05) compared with other species, with the exception of the avian species (p > 0.7). Horse presented a significantly higher fusion index than all the species tested (p < 0.05). Remarkably, ≈77% of the equine cells fused into multi-nucleated myotubes. Porcine cells achieved the lowest level of fusion with ≈ 41% of the cells fusing into myotubes. The results were evaluated using a two-sample t test. Graph shows the mean fusion index with standard deviation.