Figure 5

Dg-Dys-Syn1 regulates levels of miRNAs found in the nervous system and musculature. q-PCR analysis revealed the expression levels during all life stages and in adult body parts for (a) miR-956 (c) miR-980 and (e) miR-252. All levels have been normalized to those found in whole adult male flies. (b,d and f) LNA in situ hybridization: (b) miR-956 is present in the 3^rd instar larval brain, eye discs and body wall muscles. (d) miR-980 is present in the 3^rd instar larval brain in axons of photoreceptor cells in the optic lobe, in the eye disc, and in the midbrain. Note the reduced expression levels in miR-980 hypomorphs. miR-980 expression was not detected in the larval body wall muscles. (f) LNA in situ hybridization shows neuronal miR-252 in the optic lobe, eye disc, midbrain and ventral ganglion. Expression is not detected in the miR-252 mutant. miR-252 expression was not detected in larval body wall muscles. (g) RT q-PCR analysis of selected predicted target genes Dg, Dys, Syn1, robo and vimar in miR-252 amorphs and Dg in miR-980 hypomorphs. Three independent RNA extractions were conducted per genotype. Statistics were done using a two-tailed Student’s t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.