Figure 6From: The adhesion modulation protein, AmpA localizes to an endocytic compartment andinfluences substrate adhesion, actin polymerization and endocytosis invegetative Dictyostelium cellsAmpA is found in vesicles and in the perinuclear region but not withF-actin. A) Wt control cells (top row) and cells carrying themRFP-AmpA fusion protein construct (bottom row) were immunostained withrat anti-RFP antibody and goat anti-rat antibody conjugated to AlexaFluor 488 (Invitrogen). The left panel is anti-RFP antibody staining(green), and the right panel shows overlays of the immunostaining onDAPI stained images (blue) to mark the nucleus and transmitted images.The scale bar is 9 um. B) Western blots of cellfractionations of mRFP-AmpA expressing cells. MW is molecular weightstandard. CY-Cytoplasm, Mem-Membrane, SN-Soluble Nuclear, CB-ChromatinBound, CS-Cytoskeletal. Controls for this fractionation are in [25]. C) AmpA does not colocalize with F-actin. Cells werefixed and stained for AmpA Tap tag and F-actin. Green: Alexa fluor 488phalloidin stain for F-actin; Red: AmpA Tap tag antibody. Representativeimages are single optical sections from a Z series. Scale bar is 5 um.An AmpA-Tap-Wt (top) and an AmpA-Tap-overexpressing strain (bottom) areshown.Back to article page