mRNA expression of ob and ob-R isoforms in pressure-overloaded hearts. mRNA expression of the ob and ob-R isoforms in the normal rat heart (A). mRNA levels of ob (B), ob-Ra (C), and ob-Rb (D) isoforms in pressure-overloaded rat hearts. All quantitative PCR assay data for ob, ob-Ra, and ob-Rb mRNA is normalized by GAPDH mRNA of the same sample, and expressed as fold increase compared with the mean level of control groups at 2 or 4 weeks after surgery. 2w: control n=11, banding n=9, 4w: control n=7, banding n=8. Values are reported as means ± SD. *p<0.05 vs. control.