Table 1 Characteristics of the real-time PCR assays used in the study
Target | Species | Sequence | ACC No. | |
|---|---|---|---|---|
ob | Rat | forward | 5′-CCAAAACCCTCATCAAGACC-3′ | MN013076 |
|  |  | reverse | 5′-GTCCAACTGTTGAAGAATGTCCC-3′ |  |
ob-Ra | Rat | forward | 5′-ACACTGTTAATTTCACACCAGAG-3′ | D85557 |
|  |  | reverse | 5′-AGTCATTCAAACCATAGTTTAGG-3′ |  |
ob-Rb | Rat | forward | 5′-TCTTCTGGAGCCTGAACCCATTTC-3′ | D85558 |
|  |  | reverse | 5′-TTCTCACCAGAGGTCCCTAAACT-3′ |  |
GAPDH | Rat | forward | 5′-AACGACCCCTTCATTGAC-3′ | MN017008 |
|  |  | reverse | 5′-TCCACGACATACTCAGCAC-3′ |  |