Figure 3

Lack of PKCθ enhances protein synthesis apart from classical IRS1 signaling. A) Rates of protein synthesis determined after 4 days in differentiation media. **; P=0.0082. B) IR tyrosine phosphorylation normalized to total IR protein. **; P<0.0077. C) IRS1 tyrosine and serine phosphorylation normalized to total IRS1 protein. *; P<0.05. ***; P=0.0005. D) AKT phosphorylation normalized to total AKT protein. **; P=0.0042. E) mTOR phosphorylation normalized to total mTOR. **; P=0.0017 F) ERK1/2 phosphorylation normalized to total ERK1/2 protein. **; P=0.0023. G) ERK5 phosphorylation normalized to total ERK5 protein. Data ± SEM. n=3 for all experiments.