Figure 2

CdCl ₂ increased GADD 153 and Bak expression through ROS-generation. Level of GADD 153 was measured after CdCl₂ (12 hr) for indicated dose-dependent concentration (A). Nuclear/Cytosol fractionation is indicated GADD153 nuclear translocation by CdCl₂ (B). SH-SY5Y cells were treated with CdCl₂ (25 μM) for 12 hr following pretreatment with NAC (1 hr). Total cellular protein was extracted and separated by SDS-PAGE. Western blot analysis was performed using anti-GADD 153 or Bak or Bax. Representative western blot band for GADD153, Bak and Bax expression were shown (C). Western blot bands were quantified (D). Data are from at least three independent experiments. *p < 0.05 or **p < 0.01 vs. control.