increased Bak expression through GADD 153. Transfected with scramble RNA or GADD-153 siRNA in SH-SY5Y cells were treated with CdCl2 (25 μM) for 12 hr and then total protein was extracted and separated by SDS-PAGE. Western blot analysis was performed using anti-GADD 153 antibodies (A). SH-SY5Y cells were treated with CdCl2 (indicate dose) for 12 hr following GADD-153 siRNA transfected. Bak and GADD153 expression level (B) and apoptotic cells (C) were measured. Data are from at least three independent experiments. *p < 0.05 vs. control,#p < 0.05 vs. Cd treatment.