Figure 2
From: Retinoic acid has different effects on UCP1 expression in mouse and human adipocytes
Acute exposure of mature mouse adipocytes to ATRA induces UCP1 expression. ATRA (1 μM) was supplemented at day 8. Total RNA and protein were harvested after 24 h and analyzed by RT-qPCR and immunoblotting, respectively. Relative mRNA expression levels of FABP4, UCP1 and RARβ were determined by normalization to levels of TBP. (A) 3T3-L1. (B) WT MEFs. (C) C3H10T½. (D) Rb−/− MEFs. (E) Protein levels of UCP1 with GAPDH used as a loading control. (A-D) Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells at day 9.