Figure 3

HONE1 hybrid cells have properties of stem-like cells. A. Phase-contrast images of spheres that could be grown on the surface of monolayer epithelial cells or bottom of flask (upper right and upper left). The bottom picture shows image of parental HONE1 cell growth. The bar graph indicates that parental HONE1 cells and HONE1 hybrid cells with infected β-catenin shRNA (Hybrid/shB) do not form spheres in the same culture conditions. B. FACS analyses (bar chart, Left) show that HONE1 hybrid cells (solid bars) contain more CD24⁺, CD44⁺, CD24⁺/CD44⁺ populations than those of HONE1 cells (empty bars). The figure (right) shows that hybrid cells have increased numbers of double positive CD24⁺/CD44⁺ populations.