Figure 6

Organotypic cultures. K-HME cells form a stratified epithelium in organotypic cultures. A.B. Culture after 1 day, emerged; C. culture after 7 days, emerged. D. E-cadherin staining. E. Keratin 10, a suprabasal keratin of the epidermis. F. Involucrin (red) found in the upper strata of the epidermis and Keratin 14 (green) a basal-layer keratin. G. p63 found in the basal layer of the epidermis. H-K. Cultures were treated with ethynyl deoxyuridine (EdU) followed by a ‘click’ reaction to visualize cells that had entered S phase (green). H. Day 1 emerged; I. Day 3 emerged; J. Day 7 emerged; K. Day 3 emerged, magnified.