Figure 5
Percentage of injured HUVECs measured after exposure to neutrophil MPs and H 2 O 2 . PI-labeled HUVEC (representing injured cells) were enumerated with a fluorescence microscope. HUVEC were observed after the addition of 5 μg/mL MPs and 20 μM H2O2 with/without inhibitor (sodium azide or MPO inhibitor-I). Both inhibitors were similarly effective in protecting against cell injury. Data represent the median with interquartile range of four independent experiments. (*P < 0.05, Mann Whitney test).